Establishment and growth responses of Nile tilapia embryonic stem-like cell lines under feeder-free condition
نویسندگان
چکیده
منابع مشابه
Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers.
BACKGROUND Recently, human embryonic stem (hES) cells have become very important resources for basic research on cell replacement therapy and other medical applications. The purpose of this study was to test whether pluripotent hES cell lines could be successfully derived from frozen-thawed embryos that were destined to be discarded after 5 years in a routine human IVF-embryo transfer programme...
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Purpose: Embryonic stem (ES) cells are derived from the inner cell mass of blastocysts with self-renewal and pluripotency characteristics. These cells have potential for studies of in vitro differentiation, gene function, etc. This study was, therefore, initiated to establish new ES lines and evaluate the effects of strain on ES cell production. Materials and Methods: 3-5 day blastocysts were ...
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We generated unique porcine embryonic stem cell-like cells (pESLCs) by using inhibitors of GSK3β and MEK1. The pESLCs colonies are dome-shape morphology and show the expression of undifferentiated markers including OCT4 and NANOG. Moreover, the self-renewal capacity and morphology of the cell lines are LIFdependent, consistent with the LIF receptors expression and STAT3 phosphorylation, and con...
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embryonic stem cells (escs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. also, these cells have the self-renewing ability. escs could differentiate into most of cell lineages. lymphocytes generated from hematopoietic stem cells. we designed a feeder free culture condition to generate lymphoid lineage cells from escs. we demonstrated that il-7 plays cr...
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Due to their pluripotency and growth capability, there are great expectations for human embryonic stem cells, both as a resource for functional studies of early human development and as a renewable source of cells for use in regenerative medicine and transplantation. However, to bring human embryonic stem cells into clinical applications, their cell surface antigen expression and its chemical s...
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ژورنال
عنوان ژورنال: Development, Growth & Differentiation
سال: 2017
ISSN: 0012-1592
DOI: 10.1111/dgd.12341